Our mission
We are a team of creative, ambitious and friendly researchers who investigate membrane transport processes in pathogenic bacteria and develop innovative protein engineering tools. We combine the disciplines of structural biology, binder generation, protein biochemistry and microbiology to uncover molecular details of how membrane transport proteins expel antibiotics, shuttle siderophores and export lipids. Sybodies and NestLink enable us to target membrane transporters in the cellular context to develop rapid diagnostics, novel antibiotics and tailor-made research tools.
Fantastic work forensically analyse the effect of 1539 mutants on the function of a drug efflux pump in the context of an inward-facing #cryoem structure. Many great insights into both function and mechanism. https://t.co/WdaAuaxKWk
Our latest preprint is out🥳
https://t.co/7MfsLjMEPU
We performed DMS on EfrCD to determine the drug efflux activity profile of 1539 single variants!
Many interesting findings🔎, amongst which, that single mutations (!) can convert the exporter into a drug-specific ABC importer! https://t.co/MGRQGW0dr7
Deep mutational scan of a drug efflux pump reveals its structure-function landscape https://t.co/hfjDuJV0T1