PUBLICATIONS

MycoTransport

2023 Nat commun, 14(1):6449

Structural basis for triacylglyceride extraction from mycobacterial inner membrane by MFS transporter Rv1410

Authors: S. Remm, D. De Vecchis, J. Schöppe, C.A.J. Hutter, I. Gonda, M. Hohl, S. Newstead, L.V. Schäfer, M.A. Seeger

2022 FEMS microbiology reviews, 46(1):fuab050

Critical discussion on drug efflux in Mycobacterium tuberculosis

Authors: S. Remm, J.C. Earp, T. Dick, V. Dartois, M.A. Seeger

2020 Nature, 580(7803): p. 413-417

The ABC exporter IrtAB imports and reduces mycobacterial siderophores

Authors: F.M. Arnold, M.S. Weber, I. Gonda, M.J. Gallenito, S. Adenau, P. Egloff, I. Zimmermann, C.A.J. Hutter, L.M. Hürlimann, E.E. Peters, J. Piel, G. Meloni, O. Medalia, and M.A. Seeger*

2019 Mol Microbiol

Increased drug permeability of a stiffened mycobacterial outer membrane in cells lacking MFS transporter Rv1410 and lipoprotein LprG

Authors: M. Hohl#, S. Remm#, H.A. Eskandarian, M. Dal Molin, F.M. Arnold, L.M. Hürlimann, A. Krügel, G.E. Fantner, P. Sander, and M.A. Seeger*

2018 Sci Rep, 8.

A uniform cloning platform for mycobacterial genetics and protein production

Authors: F.M. Arnold, M. Hohl, S. Remm, H. Koliwer-Brandl, S. Adenau, S. Chusri, P. Sander, H. Hilbi, and M.A. Seeger*

DrugEfflux

2023 Nat Chem Biol, 19(4):440-450

Deep mutational scan of a drug efflux pump reveals its structure-function landscape

Authors: G. Meier, S. Thavarasah, K. Ehrenbolger, C.A.J. Hutter, L.M. Hürlimann, J. Barandun, M.A. Seeger

2022 Science Advances, 8 (41)

The ABC transporter MsbA adopts the wide inward-open conformation in E. coli cells

Authors: L. Galazzo, G. Meier, D. Januliene, K. Parey, D. De Vecchis, B. Striednig, H. Hilbi, L.V. Schäfer, I. Kuprov, A. Moeller, E. Bordignon*, M.A. Seeger*

2020 FEBS letters, 594(23):3839-3856

From in vitro towards in situ: structure-based investigation of ABC exporters by electron paramagnetic resonance spectroscopy

Authors: E. Bordignon, M.A. Seeger, L. Galazzo, G. Meier

2020 FEBS letters, 594(23):3799-3814

Structures of ABC transporters: handle with care

Authors: O. Lewinson, C. Orelle, M.A. Seeger

2020 Proc Natl Acad Sci U S A, 117(5): p. 2441-2448.

Spin-labeled nanobodies as protein conformational reporters for electron paramagnetic resonance in cellular membranes

Authors: L. Galazzo, G. Meier, M.H. Timachi, C.A.J. Hutter, M.A. Seeger*, and E. Bordignon*

2019 Nat Commun, 10(1): p. 2260

The extracellular gate shapes the energy profile of an ABC exporter

Authors: C.A.J. Hutter, M.H. Timachi, L.M. Hurlimann, I. Zimmermann, P. Egloff, H. Goddeke, S. Kucher, S. Stefanic, M. Karttunen, L.V. Schafer, E. Bordignon*, and M.A. Seeger*

2017 Elife, 6.

Exploring conformational equilibria of a heterodimeric ABC transporter

Authors: M.H. Timachi#, C.A. Hutter#, M. Hohl, T. Assafa, S. Böhm, A. Mittal, M.A. Seeger*, and E. Bordignon*

2016 Antimicrobial Agents and Chemotherapy, Aug 22;60(9):5400-11

The heterodimeric ABC transporter EfrCD mediates multidrug efflux in Enterococcus faecalis

Authors: L.M. Hürlimann, V.Corradi, M. Hohl, G.V. Bloemberg, D.P. Tieleman and M.A. Seeger*

2014 Proc Natl Acad Sci U S A, 111(30): p.11025-30

Structural basis for allosteric cross-talk between the asymmetric nucleotide binding sites of a heterodimeric ABC exporter

Authors: M. Hohl, L.M. Hürlimann, S. Böhm, J. Schöppe, M.G. Grütter, E. Bordignon, and M.A. Seeger*

2012 J Biol Chem, 287(24): p. 20395-406.

Asymmetry in the homodimeric ABC transporter MsbA recognized by a DARPin

Authors: A. Mittal, S. Böhm, M.G. Grütter, E. Bordignon*, and M.A. Seeger*

2012 Nat Struct Mol Biol, 19(4): p. 395-402.

Crystal structure of a heterodimeric ABC transporter in its inward-facing conformation

Authors: M. Hohl, C. Briand, M.G. Grütter*, and M.A. Seeger*

Sybodies

2022 Science Advances, 8 (41)

The ABC transporter MsbA adopts the wide inward-open conformation in E. coli cells

Authors: L. Galazzo, G. Meier, D. Januliene, K. Parey, D. De Vecchis, B. Striednig, H. Hilbi, L.V. Schäfer, I. Kuprov, A. Moeller, E. Bordignon*, M.A. Seeger*

2022 Trends in Biochemical Sciences, 47 (8)

Biomolecules capturing live bacteria from clinical samples

Authors: M. Sorgenfrei, L.M. Hürlimann, M.M. Remy, P.M. Keller, M.A. Seeger*

2022 Nature Communications, 13 (1):1826

Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocation

Authors: M. Botte, D. Ni, S. Schenck, I. Zimmermann, M. Chami, N. Bocquet, P. Egloff, D. Bucher, M. Trabuco, R.K.Y. Cheng, J.D. Brunner, M.A. Seeger, H. Stahlberg, M. Hennig

2022 EMBO reports, 23(4):e54199

Biparatopic sybodies neutralize SARS-CoV-2 variants of concern and mitigate drug resistance

Authors: J.D. Walter#, M. Scherer#, C.A.J. Hutter#, A.A. Garaeva#, I. Zimmermann, M. Wyss, J. Rheinberger, Y. Ruedin, J.C. Earp, P. Egloff, M. Sorgenfrei, L.M. Hürlimann, I. Gonda, G. Meier, S. Remm, S. Thavarasah, G. van Geest, R. Bruggmann, G. Zimmer, D.J. Slotboom, C. Paulino*, P. Plattet*, M.A. Seeger*

2022 Science Advances, 8(9):eabm1122

Mechanistic basis of choline import involved in teichoic acids and lipopolysaccharide modification

Authors: N. Bärland, A. Rueff, G. Cebrero, C.A.J. Hutter, M.A. Seeger, J. Veening, C. Perez

2021 Nature Communications, 12(1):5435

Allosteric modulation of LRRC8 channels by targeting their cytoplasmic domains

Authors: D. Deneka, S. Rutz, C.A.J. Hutter, M.A. Seeger, M. Sawicka, R. Dutzler

2021 Nature Communications, 12(1):4635

A synthetic nanobody targeting RBD protects hamsters from SARS-CoV-2 infection

Authors: T. Li, H. Cai, H. Yao, B. Zhou, N. Zhang, M.F. van Vlissingen, T. Kuiken, W. Han, C.H. GeurtsvanKessel, Y. Gong, Y. Zhao, Q. Shen, W. Qin, X. Tian, C. Peng, Y. Lai, Y. Wang, C.A.J. Hutter, S. Kuo, J. Bao, C. Liu, Y. Wang, A.S. Richard, H. Raoul, J. Lan, M.A. Seeger, Y. Cong, B. Rockx, G. Wong, Y. Bi, D. Lavillette, D. Li

2021 Communications biology, 4(1):751

Kinetic mechanism of Na+-coupled aspartate transport catalyzed by GltTk

Authors: G. Trinco, V. Arkhipova, A.A. Garaeva, C.A.J. Hutter, M.A. Seeger, A. Guskov, D.J. Slotboom

2021 Nature, 591(7851):677-681

Structural insights into the inhibition of glycine reuptake

Authors: A. Shahsavar, P. Stohler, G. Bourenkov, I. Zimmermann, M. Siegrist, W. Guba, E. Pinard, S. Sinning, M.A. Seeger, T.R. Schneider, R.J.P. Dawson, P. Nissen

2020 Communications biology, 3(1):753

An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification

Authors: H. Cai, H. Yao, T. Li, C.A.J. Hutter, Y. Li, Y. Tang, M.A. Seeger, D. Li

2020 FEBS letters, 594(23):3839-3856

From in vitro towards in situ: structure-based investigation of ABC exporters by electron paramagnetic resonance spectroscopy

Authors: E. Bordignon, M.A. Seeger, L. Galazzo, G. Meier

2020 Nature communications, 11(1):5588

Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2

Authors: T.F. Custódio, H. Das, D.J. Sheward, L. Hanke, S. Pazicky, J. Pieprzyk, M. Sorgenfrei, M.A. Schroer, A.Y. Gruzinov, C.M. Jeffries, M.A. Graewert, D.I. Svergun, N. Dobrev, K. Remans, M.A. Seeger, G.M. McInerney, B. Murrell, B M. Hällberg, C. Löw

2020 Nat Protoc, 15(5): p. 1707-1741

Generation of synthetic nanobodies against delicate proteins

Authors: I. Zimmermann, P. Egloff, C.A.J. Hutter, B.T. Kuhn, P. Bräuer, S. Newstead, R.J.P. Dawson, E.R. Geertsma*, and M.A. Seeger*

2020 Proc Natl Acad Sci U S A, 117(5): p. 2441-2448.

Spin-labeled nanobodies as protein conformational reporters for electron paramagnetic resonance in cellular membranes

Authors: L. Galazzo, G. Meier, M.H. Timachi, C.A.J. Hutter, M.A. Seeger*, and E. Bordignon*

2019 Nat Commun, 10(1): p. 2260

The extracellular gate shapes the energy profile of an ABC

Authors: C.A.J. Hutter, M.H. Timachi, L.M. Hurlimann, I. Zimmermann, P. Egloff, H. Goddeke, S. Kucher, S. Stefanic, M. Karttunen, L.V. Schafer, E. Bordignon*, and M.A. Seeger*

2019 Nat Methods

Engineered peptide barcodes for in-depth analyses of binding protein libraries

Authors: P. Egloff, I. Zimmermann, F.M. Arnold, C.A.J. Hutter, D. Morger, L. Opitz, L. Poveda, H.A. Keserue, C. Panse, B. Roschitzki, and M.A. Seeger*

2019 Science, 363(6431): p. 1103-1107

Structural basis for pH-dependent retrieval of ER proteins from the Golgi by the KDEL receptor

Authors: P. Bräuer, J.L. Parker, A. Gerondopoulos, I. Zimmermann, M.A. Seeger, F.A. Barr, and S. Newstead

2018 Elife, 7.

Synthetic single domain antibodies for the conformational trapping of membrane proteins

Authors: I. Zimmermann#, P. Egloff#, C.A. Hutter, F.M. Arnold, P. Stohler, N. Bocquet, M.N. Hug, S. Huber, M. Siegrist, L. Hetemann, J. Gera, S. Gmur, P. Spies, D. Gygax, E.R. Geertsma*, R.J. Dawson*, and M.A. Seeger*

NestLink

2022 Trends in Biochemical Sciences, 47 (8)

Biomolecules capturing live bacteria from clinical samples

Authors: M. Sorgenfrei, L.M. Hürlimann, M.M. Remy, P.M. Keller, M.A. Seeger*

2020 Nature, 580(7803): p. 413-417

The ABC exporter IrtAB imports and reduces mycobacterial siderophores

Authors: F.M. Arnold, M.S. Weber, I. Gonda, M.J. Gallenito, S. Adenau, P. Egloff, I. Zimmermann, C.A.J. Hutter, L.M. Hürlimann, E.E. Peters, J. Piel, G. Meloni, O. Medalia, and M.A. Seeger*

2019 Nat Methods

Engineered peptide barcodes for in-depth analyses of binding protein libraries

Authors: P. Egloff, I. Zimmermann, F.M. Arnold, C.A.J. Hutter, D. Morger, L. Opitz, L. Poveda, H.A. Keserue, C. Panse, B. Roschitzki, and M.A. Seeger*

PUBLICATIONS

MycoTransport

2023 Nat commun, 14(1):6449

Structural basis for triacylglyceride extraction from mycobacterial inner membrane by MFS transporter Rv1410

2022 FEMS microbiology reviews, 46(1):fuab050

Critical discussion on drug efflux in Mycobacterium tuberculosis

2020 Nature, 580(7803): p. 413-417

The ABC exporter IrtAB imports and reduces mycobacterial siderophores

2019 Mol Microbiol

Increased drug permeability of a stiffened mycobacterial outer membrane in cells lacking MFS transporter Rv1410 and lipoprotein LprG

2018 Sci Rep, 8.

A uniform cloning platform for mycobacterial genetics and protein production

DrugEfflux

2023 Nat Chem Biol, 19(4):440-450

Deep mutational scan of a drug efflux pump reveals its structure-function landscape

2022 Science Advances, 8 (41)

The ABC transporter MsbA adopts the wide inward-open conformation in E. coli cells

2020 FEBS letters, 594(23):3839-3856

From in vitro towards in situ: structure-based investigation of ABC exporters by electron paramagnetic resonance spectroscopy

2020 FEBS letters, 594(23):3799-3814

Structures of ABC transporters: handle with care

2020 Proc Natl Acad Sci U S A, 117(5): p. 2441-2448.

Spin-labeled nanobodies as protein conformational reporters for electron paramagnetic resonance in cellular membranes

2019 Nat Commun, 10(1): p. 2260

The extracellular gate shapes the energy profile of an ABC exporter

2017 Elife, 6.

Exploring conformational equilibria of a heterodimeric ABC transporter

2016 Antimicrobial Agents and Chemotherapy, Aug 22;60(9):5400-11

The heterodimeric ABC transporter EfrCD mediates multidrug efflux in Enterococcus faecalis

2014 Proc Natl Acad Sci U S A, 111(30): p.11025-30

Structural basis for allosteric cross-talk between the asymmetric nucleotide binding sites of a heterodimeric ABC exporter

2012 J Biol Chem, 287(24): p. 20395-406.

Asymmetry in the homodimeric ABC transporter MsbA recognized by a DARPin

2012 Nat Struct Mol Biol, 19(4): p. 395-402.

Crystal structure of a heterodimeric ABC transporter in its inward-facing conformation

Sybodies

2022 Science Advances, 8 (41)

The ABC transporter MsbA adopts the wide inward-open conformation in E. coli cells

2022 Trends in Biochemical Sciences, 47 (8)

Biomolecules capturing live bacteria from clinical samples

2022 Nature Communications, 13 (1):1826

Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocation

2022 EMBO reports, 23(4):e54199

Biparatopic sybodies neutralize SARS-CoV-2 variants of concern and mitigate drug resistance

2022 Science Advances, 8(9):eabm1122

Mechanistic basis of choline import involved in teichoic acids and lipopolysaccharide modification

2021 Nature Communications, 12(1):5435

Allosteric modulation of LRRC8 channels by targeting their cytoplasmic domains

2021 Nature Communications, 12(1):4635

A synthetic nanobody targeting RBD protects hamsters from SARS-CoV-2 infection

2021 Communications biology, 4(1):751

Kinetic mechanism of Na+-coupled aspartate transport catalyzed by GltTk

2021 Nature, 591(7851):677-681

Structural insights into the inhibition of glycine reuptake

2020 Communications biology, 3(1):753

An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification

2020 FEBS letters, 594(23):3839-3856

From in vitro towards in situ: structure-based investigation of ABC exporters by electron paramagnetic resonance spectroscopy

2020 Nature communications, 11(1):5588

Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2

2020 Nat Protoc, 15(5): p. 1707-1741

Generation of synthetic nanobodies against delicate proteins

2020 Proc Natl Acad Sci U S A, 117(5): p. 2441-2448.

Spin-labeled nanobodies as protein conformational reporters for electron paramagnetic resonance in cellular membranes

2019 Nat Commun, 10(1): p. 2260

The extracellular gate shapes the energy profile of an ABC

2019 Nat Methods

Engineered peptide barcodes for in-depth analyses of binding protein libraries

2019 Science, 363(6431): p. 1103-1107

Structural basis for pH-dependent retrieval of ER proteins from the Golgi by the KDEL receptor

2018 Elife, 7.

Synthetic single domain antibodies for the conformational trapping of membrane proteins

NestLink

2022 Trends in Biochemical Sciences, 47 (8)

Biomolecules capturing live bacteria from clinical samples

2020 Nature, 580(7803): p. 413-417

The ABC exporter IrtAB imports and reduces mycobacterial siderophores

2019 Nat Methods

Engineered peptide barcodes for in-depth analyses of binding protein libraries

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