seegerlab

PUBLICATIONS

MycoTransport

2020  Nature, 580(7803): p. 413-417

The ABC exporter IrtAB imports and reduces mycobacterial siderophores

Authors: F.M. Arnold, M.S. Weber, I. Gonda, M.J. Gallenito, S. Adenau, P. Egloff, I. Zimmermann, C.A.J. Hutter, L.M. Hürlimann, E.E. Peters, J. Piel, G. Meloni, O. Medalia, and M.A. Seeger*

2019  Mol Microbiol

Increased drug permeability of a stiffened mycobacterial outer membrane in cells lacking MFS transporter Rv1410 and lipoprotein LprG

Authors: M. Hohl#, S. Remm#, H.A. Eskandarian, M. Dal Molin, F.M. Arnold, L.M. Hürlimann, A. Krügel, G.E. Fantner, P. Sander, and M.A. Seeger*

2018  Sci Rep, 8.

A uniform cloning platform for mycobacterial genetics and protein production

Authors: F.M. Arnold, M. Hohl, S. Remm, H. Koliwer-Brandl, S. Adenau, S. Chusri, P. Sander, H. Hilbi, and M.A. Seeger*

DrugEfflux

2020  Proc Natl Acad Sci U S A, 117(5): p. 2441-2448.

Spin-labeled nanobodies as protein conformational reporters for electron paramagnetic resonance in cellular membranes

Authors: L. Galazzo, G. Meier, M.H. Timachi, C.A.J. Hutter, M.A. Seeger*, and E. Bordignon*

2019  Nat Commun, 10(1): p. 2260

The extracellular gate shapes the energy profile of an ABC exporter

Authors: C.A.J. Hutter, M.H. Timachi, L.M. Hurlimann, I. Zimmermann, P. Egloff, H. Goddeke, S. Kucher, S. Stefanic, M. Karttunen, L.V. Schafer, E. Bordignon*, and M.A. Seeger*

2017  Elife, 6.

Exploring conformational equilibria of a heterodimeric ABC transporter

Authors: M.H. Timachi#, C.A. Hutter#, M. Hohl, T. Assafa, S. Böhm, A. Mittal, M.A. Seeger*, and E. Bordignon*

2016  Antimicrobial Agents and Chemotherapy, Aug 22;60(9):5400-11

The heterodimeric ABC transporter EfrCD mediates multidrug efflux in Enterococcus faecalis

Authors: L.M. Hürlimann, V.Corradi, M. Hohl, G.V. Bloemberg, D.P. Tieleman and M.A. Seeger*

2014  Proc Natl Acad Sci U S A, 111(30): p.11025-30

Structural basis for allosteric cross-talk between the asymmetric nucleotide binding sites of a heterodimeric ABC exporter

Authors: M. Hohl, L.M. Hürlimann, S. Böhm, J. Schöppe, M.G. Grütter, E. Bordignon, and M.A. Seeger*

2012  J Biol Chem, 287(24): p. 20395-406.

Asymmetry in the homodimeric ABC transporter MsbA recognized by a DARPin

Authors: A. Mittal, S. Böhm, M.G. Grütter, E. Bordignon*, and M.A. Seeger*

2012  Nat Struct Mol Biol, 19(4): p. 395-402.

Crystal structure of a heterodimeric ABC transporter in its inward-facing conformation

Authors: M. Hohl, C. Briand, M.G. Grütter*, and M.A. Seeger*

Sybodies

2020  Nat Protoc, 15(5): p. 1707-1741

Generation of synthetic nanobodies against delicate proteins

Authors: I. Zimmermann, P. Egloff, C.A.J. Hutter, B.T. Kuhn, P. Bräuer, S. Newstead, R.J.P. Dawson, E.R. Geertsma*, and M.A. Seeger*

2020  Proc Natl Acad Sci U S A, 117(5): p. 2441-2448.

Spin-labeled nanobodies as protein conformational reporters for electron paramagnetic resonance in cellular membranes

Authors: L. Galazzo, G. Meier, M.H. Timachi, C.A.J. Hutter, M.A. Seeger*, and E. Bordignon*

2019  Nat Commun, 10(1): p. 2260

The extracellular gate shapes the energy profile of an ABC

Authors: C.A.J. Hutter, M.H. Timachi, L.M. Hurlimann, I. Zimmermann, P. Egloff, H. Goddeke, S. Kucher, S. Stefanic, M. Karttunen, L.V. Schafer, E. Bordignon*, and M.A. Seeger*

2019  Nat Methods

Engineered peptide barcodes for in-depth analyses of binding protein libraries

Authors: P. Egloff, I. Zimmermann, F.M. Arnold, C.A.J. Hutter, D. Morger, L. Opitz, L. Poveda, H.A. Keserue, C. Panse, B. Roschitzki, and M.A. Seeger*

2019  Science, 363(6431): p. 1103-1107

Structural basis for pH-dependent retrieval of ER proteins from the Golgi by the KDEL receptor

Authors: P. Bräuer, J.L. Parker, A. Gerondopoulos, I. Zimmermann, M.A. Seeger, F.A. Barr, and S. Newstead

2018  Elife, 7.

Synthetic single domain antibodies for the conformational trapping of membrane proteins

Authors: I. Zimmermann#, P. Egloff#, C.A. Hutter, F.M. Arnold, P. Stohler, N. Bocquet, M.N. Hug, S. Huber, M. Siegrist, L. Hetemann, J. Gera, S. Gmur, P. Spies, D. Gygax, E.R. Geertsma*, R.J. Dawson*, and M.A. Seeger*

NestLink

2020  Nature, 580(7803): p. 413-417

The ABC exporter IrtAB imports and reduces mycobacterial siderophores

Authors: F.M. Arnold, M.S. Weber, I. Gonda, M.J. Gallenito, S. Adenau, P. Egloff, I. Zimmermann, C.A.J. Hutter, L.M. Hürlimann, E.E. Peters, J. Piel, G. Meloni, O. Medalia, and M.A. Seeger*

2019  Nat Methods

Engineered peptide barcodes for in-depth analyses of binding protein libraries

Authors: P. Egloff, I. Zimmermann, F.M. Arnold, C.A.J. Hutter, D. Morger, L. Opitz, L. Poveda, H.A. Keserue, C. Panse, B. Roschitzki, and M.A. Seeger*