seegerlab

PUBLICATIONS

MycoTransport

2023  Nat commun, 14(1):6449

Structural basis for triacylglyceride extraction from mycobacterial inner membrane by MFS transporter Rv1410

Authors: S. RemmD. De VecchisJ. SchöppeC.A.J. HutterI. GondaM. HohlS. NewsteadL.V. SchäferM.A. Seeger

2022  FEMS microbiology reviews, 46(1):fuab050

Critical discussion on drug efflux in Mycobacterium tuberculosis

Authors: S. Remm, J.C. Earp, T. Dick, V. Dartois, M.A. Seeger

2020  Nature, 580(7803): p. 413-417

The ABC exporter IrtAB imports and reduces mycobacterial siderophores

Authors: F.M. Arnold, M.S. Weber, I. Gonda, M.J. Gallenito, S. Adenau, P. Egloff, I. Zimmermann, C.A.J. Hutter, L.M. Hürlimann, E.E. Peters, J. Piel, G. Meloni, O. Medalia, and M.A. Seeger*

2019  Mol Microbiol

Increased drug permeability of a stiffened mycobacterial outer membrane in cells lacking MFS transporter Rv1410 and lipoprotein LprG

Authors: M. Hohl#, S. Remm#, H.A. Eskandarian, M. Dal Molin, F.M. Arnold, L.M. Hürlimann, A. Krügel, G.E. Fantner, P. Sander, and M.A. Seeger*

2018  Sci Rep, 8.

A uniform cloning platform for mycobacterial genetics and protein production

Authors: F.M. Arnold, M. Hohl, S. Remm, H. Koliwer-Brandl, S. Adenau, S. Chusri, P. Sander, H. Hilbi, and M.A. Seeger*

DrugEfflux

2023  Nat Chem Biol, 19(4):440-450

Deep mutational scan of a drug efflux pump reveals its structure-function landscape

Authors: G. MeierS. ThavarasahK. EhrenbolgerC.A.J. HutterL.M. HürlimannJ. BarandunM.A. Seeger

2022  Science Advances, 8 (41)

The ABC transporter MsbA adopts the wide inward-open conformation in E. coli cells

Authors: L. Galazzo, G. Meier, D. Januliene, K. Parey, D. De Vecchis, B. Striednig, H. Hilbi, L.V. Schäfer, I. Kuprov, A. Moeller, E. Bordignon*, M.A. Seeger*

2020  FEBS letters, 594(23):3839-3856

From in vitro towards in situ: structure-based investigation of ABC exporters by electron paramagnetic resonance spectroscopy

Authors: E. Bordignon, M.A. Seeger, L. Galazzo, G. Meier

2020  FEBS letters, 594(23):3799-3814

Structures of ABC transporters: handle with care

Authors: O. Lewinson, C. Orelle, M.A. Seeger

2020  Proc Natl Acad Sci U S A, 117(5): p. 2441-2448.

Spin-labeled nanobodies as protein conformational reporters for electron paramagnetic resonance in cellular membranes

Authors: L. Galazzo, G. Meier, M.H. Timachi, C.A.J. Hutter, M.A. Seeger*, and E. Bordignon*

2019  Nat Commun, 10(1): p. 2260

The extracellular gate shapes the energy profile of an ABC exporter

Authors: C.A.J. Hutter, M.H. Timachi, L.M. Hurlimann, I. Zimmermann, P. Egloff, H. Goddeke, S. Kucher, S. Stefanic, M. Karttunen, L.V. Schafer, E. Bordignon*, and M.A. Seeger*

2017  Elife, 6.

Exploring conformational equilibria of a heterodimeric ABC transporter

Authors: M.H. Timachi#, C.A. Hutter#, M. Hohl, T. Assafa, S. Böhm, A. Mittal, M.A. Seeger*, and E. Bordignon*

2016  Antimicrobial Agents and Chemotherapy, Aug 22;60(9):5400-11

The heterodimeric ABC transporter EfrCD mediates multidrug efflux in Enterococcus faecalis

Authors: L.M. Hürlimann, V.Corradi, M. Hohl, G.V. Bloemberg, D.P. Tieleman and M.A. Seeger*

2014  Proc Natl Acad Sci U S A, 111(30): p.11025-30

Structural basis for allosteric cross-talk between the asymmetric nucleotide binding sites of a heterodimeric ABC exporter

Authors: M. Hohl, L.M. Hürlimann, S. Böhm, J. Schöppe, M.G. Grütter, E. Bordignon, and M.A. Seeger*

2012  J Biol Chem, 287(24): p. 20395-406.

Asymmetry in the homodimeric ABC transporter MsbA recognized by a DARPin

Authors: A. Mittal, S. Böhm, M.G. Grütter, E. Bordignon*, and M.A. Seeger*

2012  Nat Struct Mol Biol, 19(4): p. 395-402.

Crystal structure of a heterodimeric ABC transporter in its inward-facing conformation

Authors: M. Hohl, C. Briand, M.G. Grütter*, and M.A. Seeger*

Sybodies

2022  Science Advances, 8 (41)

The ABC transporter MsbA adopts the wide inward-open conformation in E. coli cells

Authors: L. Galazzo, G. Meier, D. Januliene, K. Parey, D. De Vecchis, B. Striednig, H. Hilbi, L.V. Schäfer, I. Kuprov, A. Moeller, E. Bordignon*, M.A. Seeger*

2022  Trends in Biochemical Sciences, 47 (8)

Biomolecules capturing live bacteria from clinical samples

Authors: M. Sorgenfrei, L.M. Hürlimann, M.M. Remy, P.M. Keller, M.A. Seeger*

2022  Nature Communications, 13 (1):1826

Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocation

Authors: M. Botte, D. Ni, S. Schenck, I. Zimmermann, M. Chami, N. Bocquet, P. Egloff, D. Bucher, M. Trabuco, R.K.Y. Cheng, J.D. Brunner, M.A. Seeger, H. Stahlberg, M. Hennig

2022  EMBO reports, 23(4):e54199

Biparatopic sybodies neutralize SARS-CoV-2 variants of concern and mitigate drug resistance

Authors: J.D. Walter#, M. Scherer#, C.A.J. Hutter#, A.A. Garaeva#, I. Zimmermann, M. Wyss, J. Rheinberger, Y. Ruedin, J.C. Earp, P. Egloff, M. Sorgenfrei, L.M. Hürlimann, I. Gonda, G. Meier, S. Remm, S. Thavarasah, G. van Geest, R. Bruggmann, G. Zimmer, D.J. Slotboom, C. Paulino*, P. Plattet*, M.A. Seeger*

2022  Science Advances, 8(9):eabm1122

Mechanistic basis of choline import involved in teichoic acids and lipopolysaccharide modification

Authors: N. Bärland, A. Rueff, G. Cebrero, C.A.J. Hutter, M.A. Seeger, J. Veening, C. Perez

2021  Nature Communications, 12(1):5435

Allosteric modulation of LRRC8 channels by targeting their cytoplasmic domains

Authors: D. Deneka, S. Rutz, C.A.J. Hutter, M.A. Seeger, M. Sawicka, R. Dutzler

2021  Nature Communications, 12(1):4635

A synthetic nanobody targeting RBD protects hamsters from SARS-CoV-2 infection

Authors: T. Li, H. Cai, H. Yao, B. Zhou, N. Zhang, M.F. van Vlissingen, T. Kuiken, W. Han, C.H. GeurtsvanKessel, Y. Gong, Y. Zhao, Q. Shen, W. Qin, X. Tian, C. Peng, Y. Lai, Y. Wang, C.A.J. Hutter, S. Kuo, J. Bao, C. Liu, Y. Wang, A.S. Richard, H. Raoul, J. Lan, M.A. Seeger, Y. Cong, B. Rockx, G. Wong, Y. Bi, D. Lavillette, D. Li

2021  Communications biology, 4(1):751

Kinetic mechanism of Na+-coupled aspartate transport catalyzed by GltTk

Authors: G. Trinco, V. Arkhipova, A.A. Garaeva, C.A.J. Hutter, M.A. Seeger, A. Guskov, D.J. Slotboom

2021  Nature, 591(7851):677-681

Structural insights into the inhibition of glycine reuptake

Authors: A. Shahsavar, P. Stohler, G. Bourenkov, I. Zimmermann, M. Siegrist, W. Guba, E. Pinard, S. Sinning, M.A. Seeger, T.R. Schneider, R.J.P. Dawson, P. Nissen

2020  Communications biology, 3(1):753

An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification

Authors: H. Cai, H. Yao, T. Li, C.A.J. Hutter, Y. Li, Y. Tang, M.A. Seeger, D. Li

2020  FEBS letters, 594(23):3839-3856

From in vitro towards in situ: structure-based investigation of ABC exporters by electron paramagnetic resonance spectroscopy

Authors: E. Bordignon, M.A. Seeger, L. Galazzo, G. Meier

2020  Nature communications, 11(1):5588

Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2

Authors: T.F. Custódio, H. Das, D.J. Sheward, L. Hanke, S. Pazicky, J. Pieprzyk, M. Sorgenfrei, M.A. Schroer, A.Y. Gruzinov, C.M. Jeffries, M.A. Graewert, D.I. Svergun, N. Dobrev, K. Remans, M.A. Seeger, G.M. McInerney, B. Murrell, B M. Hällberg, C. Löw

2020  Nat Protoc, 15(5): p. 1707-1741

Generation of synthetic nanobodies against delicate proteins

Authors: I. Zimmermann, P. Egloff, C.A.J. Hutter, B.T. Kuhn, P. Bräuer, S. Newstead, R.J.P. Dawson, E.R. Geertsma*, and M.A. Seeger*

2020  Proc Natl Acad Sci U S A, 117(5): p. 2441-2448.

Spin-labeled nanobodies as protein conformational reporters for electron paramagnetic resonance in cellular membranes

Authors: L. Galazzo, G. Meier, M.H. Timachi, C.A.J. Hutter, M.A. Seeger*, and E. Bordignon*

2019  Nat Commun, 10(1): p. 2260

The extracellular gate shapes the energy profile of an ABC

Authors: C.A.J. Hutter, M.H. Timachi, L.M. Hurlimann, I. Zimmermann, P. Egloff, H. Goddeke, S. Kucher, S. Stefanic, M. Karttunen, L.V. Schafer, E. Bordignon*, and M.A. Seeger*

2019  Nat Methods

Engineered peptide barcodes for in-depth analyses of binding protein libraries

Authors: P. Egloff, I. Zimmermann, F.M. Arnold, C.A.J. Hutter, D. Morger, L. Opitz, L. Poveda, H.A. Keserue, C. Panse, B. Roschitzki, and M.A. Seeger*

2019  Science, 363(6431): p. 1103-1107

Structural basis for pH-dependent retrieval of ER proteins from the Golgi by the KDEL receptor

Authors: P. Bräuer, J.L. Parker, A. Gerondopoulos, I. Zimmermann, M.A. Seeger, F.A. Barr, and S. Newstead

2018  Elife, 7.

Synthetic single domain antibodies for the conformational trapping of membrane proteins

Authors: I. Zimmermann#, P. Egloff#, C.A. Hutter, F.M. Arnold, P. Stohler, N. Bocquet, M.N. Hug, S. Huber, M. Siegrist, L. Hetemann, J. Gera, S. Gmur, P. Spies, D. Gygax, E.R. Geertsma*, R.J. Dawson*, and M.A. Seeger*

NestLink

2022  Trends in Biochemical Sciences, 47 (8)

Biomolecules capturing live bacteria from clinical samples

Authors: M. Sorgenfrei, L.M. Hürlimann, M.M. Remy, P.M. Keller, M.A. Seeger*

2020  Nature, 580(7803): p. 413-417

The ABC exporter IrtAB imports and reduces mycobacterial siderophores

Authors: F.M. Arnold, M.S. Weber, I. Gonda, M.J. Gallenito, S. Adenau, P. Egloff, I. Zimmermann, C.A.J. Hutter, L.M. Hürlimann, E.E. Peters, J. Piel, G. Meloni, O. Medalia, and M.A. Seeger*

2019  Nat Methods

Engineered peptide barcodes for in-depth analyses of binding protein libraries

Authors: P. Egloff, I. Zimmermann, F.M. Arnold, C.A.J. Hutter, D. Morger, L. Opitz, L. Poveda, H.A. Keserue, C. Panse, B. Roschitzki, and M.A. Seeger*